Review basics of enzymatic assays for hts assay guidance manual. Assays are performed using radioactive labels that emit electrons with only a short range about 10 m in water. Assays are performed using radioactive labels that emit electrons with only a short range about 10 um in water. Pdf scintillation proximity assays in highthroughput. Abstract scintillation proximity assay spa is a versatile homogeneous technique for radioactive assays which eliminates the need for. It has also been validated in the evaluation of proteinpeptide interactions, proteindna interactions, and cellular adhesion molecule binding. Flashplate or microbead as addition of free biotin was able to block this. When bound close to a solid scintillator surface by the binding reaction, they are able. Development of a complex scintillation proximity assay for high. Scintillation proximity assay spa is a technology whereby binding reactions can be assayed without the washing or filtration procedures normally used to separate bound from free fractions. The approach involves the use of isolated phagosomes containing scintillant latex beads and lysosomes labeled with a tritiated marker. Pdf scintillation proximity assays spas have become a powerful tool for highthroughput. The type of beads that are involved in the spa are microscopic in size and within the beads itself, there is a scintillant which emits light when it is stimulated. Pdf version of this page 735k pdf version of this title 33m similar articles in pubmed.
Fluomicrospheres coated with antibodies or receptors eliminate the need to separate bound from free ligand in both radioimmunoassays and. Free 3hctp, not bound to the spa beads, does not stimulate photon. It does not involve a filtration step to separate bound from free ligand as is the case in a traditional receptorbinding assay. Combine the sensitivity of radioactive assays with the convenience of a homogeneous no wash steps assay using spa technology. Pdf scintillation proximity assays in highthroughput screening. Pdf scintillation proximity assays spas have become a powerful tool for high throughput. Pdf scintillation proximity assay spa as a new approach to. Scintillation proximity assay wiley online library. The technology is homogeneous in nature, as it relies on a mix and measure format and avoids a filtration step to separate bound from unbound radiolabeled ligand as is the case in a traditional receptorbinding assay.
Scintillation proximity assay spa is a radioisotopic technology format used to measure a wide range of biological interactions, including drugtarget binding affinity studies. Scintillation proximity assay listed as spa looking for abbreviations of spa. Scintillation proximity assays spa are a beadbased assay technology for radioligand binding studies in drug research 1, 2. Fluomicrospheres coated with antibodies or receptors eliminate the need to separate bound from free ligand in both radioimmunoassays and ligandbinding assays. A cellfree scintillation proximity assay for studies on. Use of scintillation proximity assay to measure radioligand binding. Protocol 1, the amount of wga spa beads see support protocol 2, and. Scintillation proximity assay spa technology to study. Request pdf a scintillation proximity assay for rna detection a homogeneous scintillation proximity assay spa for detection of rna transcripts is described. Free ligand does not need to be physically separated from bound ligand for. Spa is a beadbased assay technique that has been applied to radioimmunoassays, receptorbinding assays and enzyme assays. Scintillation proximity assay how is scintillation. Scintillation proximity assay on the topcount microplate.
Scintillation proximity assay spa as a new approach to. It is a homogenous assay format and therefore does not require the classical physical. We describe a cell free scintillation proximity assay developed to study the mechanisms of lysosome targeting to phagosomes and the regulation of this process by igg. We describe a cellfree scintillation proximity assay developed to study the mechanisms of lysosome targeting to phagosomes and the regulation of this process by igg. Pdf scintillation proximity assay spa is a radioisotopic technology. Scintillation proximity assay spa is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes including use with enzyme targets and receptor targets, radioimmunoassays, and molecular interactions. Scintillation proximity assay an overview sciencedirect topics. Scintillation proximity assay spa as a new approach to determine. Scintillation proximity assays in highthroughput screening. Scintillation proximity assay spa is a technology whereby binding reactions can be. The assay is homogeneous in nature, as it relies on a mix and measure format.